Labels:text | font | black and white | screenshot | white OCR: The complementation test is the standard way to determine experimentally whether two mutations affect the same or different genes. As illustrated, the test involves intercrossing the two mutants in question and examining the phenotype of the heterozygous offspring. If the phenotype is mutant, the two mutations do not complement one another and therefore affect the same gene. If the phenotype is normal, the two mutations do complement each other and therefore affect different genes. The biochemical rationale of a complementation test is explained in the illustration.
